The determining the photoluminescence intensity for assessing of the small bowel viability in the experiment

Abstract

Aims: Explore the potential of utilising photoluminescence intensity (PhLI) indicators for evaluating bowel viability.

Methodology: PhLI of the small bowel walls on the initial, middle, and distal parts were measured in the control group (10 rats). The loop of the initial (10 rats), middle (10 rats) and distal (10 rats) part of the bowel with the mesentery was ligated. PhLI of the bowel walls were measured on the afferent (AP), efferent (EP) and ligated (LP) parts in 6 hours. LGN-503 argon laser with a wavelength of 458 nm and a power of 200 mW was used. Sections of bowels were taken for histological examinations.

Results:  In AP revealed dystrophic disorders, in EP revealed minor changes, in LP revealed necrosis. The small bowel wall PhLI with altered viability decreased at the wavelength range λ = 470-490 nm, but the absolute indicators were significantly variable. The ratio of PhLI at wavelengths λ = 474 nm/λ = 489 nm on the initial, middle, distal bowel's parts didn’t significantly differ in control. The ratio on each bowel part didn’t significantly differ on the AP, EP, LP. The ratio's differences of the viable and non-viable bowels were significant on each part.

Conclusion: 1. The small bowel wall PhLI with altered viability decreases at the wavelength range λ = 470-490 nm. 2. The ratio of the small bowel walls PhLI indicators at the wavelengths λ=474/λ=489 nm is statistically significantly different in the bowel parts without altered viability and the bowel parts with necrosis. 3. Indicators of the ratio > 17 units testify the absence of viability altered, indicators in the range of 13-17 units testify the morphological disorders without necrosis, indicators < 13 units testify the bowel necrosis. 4. The determination of this ratio is promising for use in patients to assess its informativeness.